Tswana
Classification: Niger-Congo, Atlantic-Congo, Volta-Congo, Benue-Congo, Bantoid, Southern, Narrow Bantu, Central, S, Sotho-Tswana (S.30), Tswana. Around 70% of the population speak Tswana (also known as Setswana). Some official business is conducted in Tswana, the media often use English and Tswana interchangeably, and it's a lingua franca between different citizens who don't speak English.

Tswapong
Classification: Niger-Congo, Atlantic-Congo, Volta-Congo, Benue-Congo, Bantoid, Southern, Narrow Bantu, Central, S, Sotho-Tswana (S.30), Tswapong. Several thousand speakers, in the Central District and Mahalapye Sub-district.

Kagalagadi
Classification: Niger-Congo, Atlantic-Congo, Volta-Congo, Benue-Congo, Bantoid, Southern, Narrow Bantu, Central, S, Sotho-Tswana (S.30), Kgalagadi. Estimated total of about 35,000 speakers in Botswana.

Birwa
Classification: Niger-Congo, Atlantic-Congo, Volta-Congo, Benue-Congo, Bantoid, Southern, Narrow Bantu, Central, S, Sotho-Tswana (S.30), Sotho. Estimated total of about 10,000 speakers in Botswana.

Kalanga
Classification: Niger-Congo, Atlantic-Congo, Volta-Congo, Benue-Congo, Bantoid, Southern, Narrow Bantu, Central, S, Shona (S10). Estimated total of about 160,000 speakers in Botswana.

Ndbele
Classification: Niger-Congo, Atlantic-Congo, Volta-Congo, Benue-Congo, Bantoid, Southern, Narrow Bantu, Central, S, Ngui. Estimated total of about 10,000 speakers in the Northeast District, though this is the main language over the border in Zimbabwe's Matabele Province.

Herero
Classification: Niger-Congo, Atlantic-Congo, Volta-Congo, Benue-Congo, Bantoid, Southern, Narrow Bantu, Central, R, Herero. Estimated total of about 31,000 speakers scattered among other ethnic groups, often having their own area in towns and villages. This is one of Namibia's major ethnic groups.

Yeyi
Classification: Niger-Congo, Atlantic-Congo, Volta-Congo, Benue-Congo, Bantoid, Southern, Narrow Bantu, Central, R, Yeye. Estimated total of about 27,000 speakers in Botswana, with probably another 20,000 ethnic Bayeyi who do not actually speak Yeyi.
Mbukushu
Classification: Niger-Congo, Atlantic-Congo, Volta-Congo, Benue-Congo, Bantoid, Southern, Narrow Bantu, Central, K, Kwangwa. Estimated total of about 12,000 speakers in Botswana, located in the Northwest District, especially in Gomare, and in the villages to the north of there, and in the Okavango Delta.

Subiya
Classification: Niger-Congo, Atlantic-Congo, Volta-Congo, Benue-Congo, Bantoid, Southern, Narrow Bantu, Central, K, Subia (L50). Estimated total of about 12,000 speakers in Botswana, mostly living in the Northwest and Chobe Districts.